Poster 113: A Comparison of Wound Healing Processes Between Ex Vivo Produced Oral Mucosa Equivalent Transplantation and Artificial Dermis Transplantation: The Influence of Keratinocytes on Biological Events in the Lamina Propria

Abstract

For clinical application we fabricated ex vivo produced oral mucosa equivalent (EVPOME) from patient oral keratinocytes, completely excluding both heterogenic protein interaction and xenoplantation. This study was aimed at comparing artificial dermis and EVPOME transplantations on wound healing mechanisms. 1Analyses of fibroblast dynamics (1), mast cell expression (2), nerve regeneration (3), and revascularization (4) of EVPOME and artificial dermis transplantations were carried out at the Department of Oral and Maxillofacial Surgery, Kobe University Hospital.2Using an acute tongue wound model on rats, the relation between keratinocyte regeneration and biological events in the lamina propria were immunohistochemically analyzed. 1Immunohistochemistry2Clinical investigation3Statistical analysis 1The keratinocyte-induced neuromucosal system was involved in the promotion of wound healing after EVPOME transplantation and induced faster regeneration of wounds than after artificial dermis transplantation.2Although the period of time for complete epithelialization by artificial dermis did not differ from that by EVPOME, monitoring of nerve and vascular regeneration demonstrated that tissues of the transplanted artificial dermis were incomplete and still in the process of regeneration.3With EVPOME, fibroblast migration was inhibited because of the dense structure of the acellular dermal matrix, and the wound healing process by the keratinocyte-derived cytokine system was manifest predominantly in the early stages after transplantation. The keratinocytes and the acellular dermal matrix of EVPOME are involved in a variety of biological events and are considered to induce faster wound healing, a feature that is useful in clinical applications.