Abstract
The development of cryoprotective serum-free or xeno-free media is required for safe use of cryopreserved testicular material for transplantation. In this study, the solutions containing 10% fetal bovine serum (FBS) or 5 mg/ml bovine serum albumin (BSA) did not significantly enhance the general survival of interstitial cells (ICs) after cryopreservation but increase their metabolic activity and steroid producing cell (HSD+-cells) survival. The use of 50 and 100 mg/ml hydroxyethyl starch (HES) in DMSO-based cryoprotective solutions instead of BSA or FBS enabled the improvement of the IC general survival and the survival of HSD+-cells. The use of HES supplemented media allowed to decrease the dimethyl sulfoxide (DMSO) concentration from 1.4 to 0.7 M and to preserve the amount and metabolic activity of ICs. Thus, designing cryoprotective media containing DMSO and HES can facilitate the formulation of serum-free solutions for cryopreservation that in turn paves a way for implementation of the use of cryopreserved material for practical medicine.
Highlights
The development of cryoprotective serum- free or xeno-free media, is required for safe use of cryopreserved testicular material for transplantation
Our findings showed the dimethyl sulfoxide (DMSO) supplement to increase significantly the IСs survival (Fig. 1)
The general interstitial cells (ICs) survival was very low without DMSO use, but its maximum concentration (2.8 M) hardly promoted survival as well, and even suppressed it when applying the additive-free solutions and those containing bovine serum albumin (BSA), fetal bovine serum (FBS) or hydroxyethyl starch (HES)
Summary
The development of cryoprotective serum- free or xeno-free media, is required for safe use of cryopreserved testicular material for transplantation. The solutions containing 10% fetal bovine serum (FBS) or 5 mg/ml bovine serum albumin (BSA) did not significantly enhance the general survival of interstitial cells (ICs) after cryopreservation, but increase their metabolic activity and steroid producing cell (HSD+-cells) survival. Liu. Testicular interstitial cells (ICs) have a key role in ensuring the reproductive function in mammals by producing male sex hormones. Testicular interstitial cells (ICs) have a key role in ensuring the reproductive function in mammals by producing male sex hormones These properties stipulate the value of ICs for a potential application in regenerative medicine [8, 18]. Liuta et al [23] have suggested that serum 'stabilizes' membranes, prevents their osmotic damage, and protects cells against
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