Abstract
We tested whether postconditioning limited infarct size in rabbits with haemodynamic nitrate tolerance. Male rabbits made tolerant (tol.) to the hypotensive response to 30 μg/kg intravenous nitroglycerin (NG) by a preceding 1-week exposure to transdermal NG (0.07 mg/kg/h) were subjected to 35 min coronary occlusion (test ischaemia) followed by 3 h of reperfusion with the following additional interventions: no intervention (NI); postconditioning pacing (PPC): five cycles of 5-min periods of rapid ventricular pacing (500 b.p.m.) or postconditioning coronary occlusion (PCO): five cycles of 5-min coronary occlusion with 10-min interpacing/inter-occlusion intervals, applied after the end of the test ischemia. These protocols was applied in both nitrate tolerant and non-tolerant animals. Infarct size expressed as a percentage of area at risk (I/R) was determined by triphenyltetrazolium chloride staining, left ventricular cyclic nucleotides (pmol/mg ww) were determined by radioimmunoassay from samples out of the area at risk, 75 min after the test ischaemia. The results obtained are seen in the table:
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