Post-Transfusions-Lebensdauer und Verträglichkeit von Hydroxyethylstärke(HES)-kryokonservierten Erythrozyten – Erfahrungen am autologen Transfusionsmodell Hund

Abstract

Experiments were carried out with dog erythrocytes as an animal model for human erythrocytes. Caused by hemolysis 30% of the erythrocytes formerly cryopreserved with hydroxyethyl starch (HES) (200000/0.5; 12,5%) could not be labeled with <sup>51</sup>Cr. All the cryopreserved labeled erythrocytes showed the same in vivo survival as labeled fresh red cells. After autologous substitution of 10% of the dog’s blood by the HES cryopreserve, the HES plasma level decreased, following a first-order kinetics with t<sub>½</sub> = 4 h. Concerning the blood flow behavior, a transient reduction by the cryopreserved erythrocytes was seen, while the HES had adverse as well as beneficial effects. By further improvement the preserve should become suitable for clinical application.