Abstract
WE have shown previously1 that poly(A)-containing RNA can be isolated from the green alga Chlorella fusca 8p (ref. 2) and that this RNA has messenger-like properties, as it is polydisperse and stimulates polypeptide synthesis in vitro in a wheat embryo system. We show here that the Chlorella protein isocitrate lyase has been identified as a product of in vitro translation of poly(A)-containing RNA, so confirming messenger function in this fraction. We have used the in vitro translation system as a means of assaying for the presence of isocitrate lyase message sequences in poly(A)-containing RNA isolated from Chlorella cultured under different conditions, and we report here that slow-growing non-induced cells may contain high levels of mRNA for this enzyme although the enzyme is not synthesised.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
