Abstract
Present investigation was conducted to determine the post-thaw sperm motility and acrosomal damage of filtered and non-filtered frozen semen of Murrah buffalo bulls. Twenty semen ejaculates (from four Murrah buffalo bulls collected at weekly interval) were diluted in Tris egg yolk glycerol extender and divided into two parts. One was filtered through sephadex G-100 column and the other portion was kept as such (non-filtered). Both fractions were frozen in liquid nitrogen (-196°C) by the standard method developed in the laboratory. After 24 h of freezing, non-filtered and filtered semen samples were thawed at 37°C for 1 min. These samples were incubated at 37°C in a water both. The different seminal characteristics i.e. percent progressive sperm motility, live and abnormal spermatozoa and spermatozoa with damaged acrosome were assessed at hourly interval till they remained motile. The filtered frozen and thawed semen showed significantly (p<0.05) high sperm viability and acrosomal integrity as compared to non-filtered semen. (Asian-Aust. J. Anim. Sci. 2003. Vol 16, No. 10 : 1424-1428)
Highlights
The Murrah buffalo is the most famous breed of domestic Indian buffalo and one of the most efficient milk as well as butter fat producing animals
The variation in percent progressive sperm motility of semen observed in the present study was because of environmental and nutritional status of the bulls
The seminal characteristics obtained in the present study were within the range of values reported by other workers (Raizada, 1979; Dhami, et al, 1992; Goyal, 1993; Panghal, 1996; Younis et al, 1998)
Summary
The Murrah buffalo is the most famous breed of domestic Indian buffalo and one of the most efficient milk as well as butter fat producing animals. The success of artificial insemination technique in buffalo is associated with effective prolongation of fertile life of spermatozoa obtained from genetically superior bulls under in vitro storage condition. Extensive research work has been carried out both in India and abroad on various aspects of improvement in the freezing technology of buffalo bull semen but still the post-thaw semen quality is not as good as that of bull semen (Dhami, et al, 1995). A high percentage of abnormal and dead spermatozoa in bull (Shannon and Curson, 1972) and buffalo bull semen (Heuer and Tahir, 1982) exert toxic and lytic effect on motile sperm cells in the ejaculate, which leads to lower fertility. The removal of nonmotile, dead and abnormal spermatozoa from the semen by filtration before freezing, may results in higher post-thaw motility as compared to non-filtered semen
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