Post-synthesis of covalent organic frameworks as a hydrophilic platform for specific detection of egg ovalbumin under physiological pH

Abstract

Ovalbumin (OVA) is an important protein source in our daily life. Unfortunately, the food safety problem has become more and more serious, such as protein allergy and contaminated protein. Therefore, it is necessary to detect vital proteins efficiently and rapidly. Mass spectrometry (MS) is a powerful tool for the detection of proteins. Herein, dual amino acids functionalized covalent organic frameworks containing disulfide covalent bonds (COF@SS@GC, where G is glutathione and C is cysteine) were facilely prepared for OVA enrichment through hydrophilic interaction liquid chromatography (HILIC) under physiological pH. The results showed that COF@SS@GC had displayed sensitive detection (0.1 fmol), good selectivity (OVA: BSA = 1:100), adsorption capacity (311 mg/g), stability, reproducibility, linearity, LOQ level (42 μg/mL) and recovery ratio (64.83 %) for OVA. COF@SS@GC also demonstrated satisfactory purification ability in the enrichment of egg white, indicating that COF@SS@GC had great potential in the enrichment of protein from complex samples.