Abstract
The size of the DNA synthetized after treatment of an excision defective E. coli strain with cis-dichlorodiammineplatinum(II) ( cis-PDD) was examinated using sedimentation in alkaline sucrose gradients. DNA synthetized during a 10 minutes pulse after treatment with cis-PDD sediments with a molecular weight lower than control DNA from untreated cells. Post treatment incubation of the cells leads to an increase in the sedimentation rate of this DNA which approaches that of normal DNA. This last process is partially abolished in a uvr B5 rec B21 double mutant. These results suggest that single strand breaks or gaps are produced during treatment and are filled in during further reincubation as part of a post replication repair process.
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