Post - prandial rise of microvesicles in peripheral blood of healthy human donors

Vid Šuštar,Veronika Kralj-Iglič,Roman Štukelj,Eva Ogorevc,Apolonija Bedina-Zavec,Rado Janša,Peter Veranič,Mojca Frank,Keriya Mam

doi:10.1186/1476-511x-10-47

Abstract

BackgroundMicrovesicles isolated from body fluids are membrane - enclosed fragments of cell interior which carry information on the status of the organism. It is yet unclear how metabolism affects the number and composition of microvesicles in isolates from the peripheral blood.AimTo study the post - prandial effect on microvesicles in isolates from the peripheral blood of 21 healthy donors, in relation to blood cholesterol and blood glucose concentrations.ResultsThe average number of microvesicles in the isolates increased 5 hours post - prandially by 52%; the increase was statistically significant (p = 0.01) with the power P = 0.68, while the average total blood cholesterol concentration, average low density lipoprotein cholesterol concentration (LDL-C) and average high density lipoprotein cholesterol concentration (HDL-C) all remained within 2% of their fasting values. We found an 11% increase in triglycerides (p = 0.12) and a 6% decrease in blood glucose (p < 0.01, P = 0.74). The post - prandial number of microvesicles negatively correlated with the post - fasting total cholesterol concentration (r = - 0.46, p = 0.035) while the difference in the number of microvesicles in the isolates between post - prandial and post - fasting states negatively correlated with the respective difference in blood glucose concentration (r = - 0.39, p = 0.05).ConclusionsIn a population of healthy human subjects the number of microvesicles in isolates from peripheral blood increased in the post - prandial state. The increase in the number of microvesicles was affected by the fasting concentration of cholesterol and correlated with the decrease in blood glucose.

Highlights

Microvesicles isolated from body fluids are membrane - enclosed fragments of cell interior which carry information on the status of the organism
The concentration of high density lipoprotein cholesterol concentration (HDL-C) was higher in the female population ((1.62 ± 0.22) mmol/L) than in the male population ((1.52 ± 0.15) mmol/L)
We found a 6% decrease in the blood glucose concentration (p < 0.01) with the power P = 0.74 at a = 0.05 (Table 3)

Summary

Introduction

Microvesicles isolated from body fluids are membrane - enclosed fragments of cell interior which carry information on the status of the organism. In the final stage of the complex process of membrane budding [1,2,3,4], buds are pinched off from the cell membrane to become microvesicles (MVs) These submicron - sized membrane - enclosed fragments of cell interior [5,6], which contain biologically important molecules (such as nucleic acids and proteins), reflect the composition of the mother cell [7,8]. They can move with body fluids and are able to reach distal cells. MVs may carry organelles and viruses [8] and are involved in spreading

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