Abstract
At present the Cantabric Brown Bear (Ursus arctos), which probably constitutes the last pure breed aggregate of brown bear in the world, is a seriously endangered population (~80 animals are living in a fragmentary area in the North of Spain). The harvesting of gametes obtained post-mortem could be a useful tool in creating a genetic resource bank. The present work is a preliminary report about post-mortem spermatozoa (spz) recovery in a Cantabric Brown Bear (7 years old, 170 kg), died 8 days after an accident in the wilds (3 rd May 1998). The testis was extracted 70 min post-mortem and the epididymis was dissected at 5°C. A sample of gametes was obtained from each epididymis region and deferens ductus (reference of the preejaculatory spermatic cells). The percentage and position of the cytoplasmic droplets (CD) were evaluated as a gamete maturity index (viability prediction).
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