Abstract
Here we evaluate the effects of different concentrations of the antibiotic ampicillin on the growth and development of Chrysomya putoria. Third-generation, first instar larvae (L1) reared on 60 grams of homogenate+agar 65% were treated with ampicillin sodium. The experiment consisted of four replicates (40 larvae/replicate) of each antibiotic concentration tested (T1: 466µg/mL ; T2: 81.33 mg/mL and T3: 166.66mg/mL) and a T4: control. The body mass of the mature larvae, after they abandoned the diet, were recorded in batches of five. The variation between the mean body mass of larvae and the duration of larval and pupal stages, and overall duration of the development, viability and normal rates were analyzed by ANOVA. There were no significant differences between the four treatments in the following parameters: body mass of larvae that discontinued the diet as well as the duration of larval, pupal, and total development. The sex ratios found in the four treatments did not differ from those expected. Normality rates were 100% for all treatments. There were no significant differences between treatments for larval and overall viability, but pupal viability differed significantly between T1 and the control, T1 and T2, and between the control and T3. The antibiotic did not appear to significantly alter the development of C. putoria.
Highlights
Considering patients who undergo an antibiotic regimen or cases of overuse of antibiotics, and the fact that we know little about how and for how long fly larvae accumulate these drugs in their system, how they eliminate them and how drugs affect their development (Soto 2008), we endeavored to investigate the effects of ampicillin on the postembryonic development of Chrysomya putoria (Wiedemann, 1830)
Adult blowflies (C. putoria) were obtained from a stock colony originated from insects collected at the Rio de Janeiro Zoo, which is located at the Quinta da Boa Vista Park, São Cristóvão, RJ
Human myiasis is the presence of fly larvae in human tissues, where they feed and develop as parasites
Summary
It has high synanthropy and can be found in several biotopes, including rural, urban and anthropic forest environments (Ferraz et al 2009, Batista-da-Silva et al 2010, Kosmann et al 2013, Cabrini et al 2013) This species has been reported to cause secondary myiasis in animals and humans (Guimaraes and Pavavero 1999). The species of Calliphoridae can feed on human tissues during their larval stage, either on dead tissues, (participating in the process of corpse decomposition), on live tissues, (causing myiasis) or during larval therapy. These species can absorb drugs from host tissues, which can lead to changes in their development rate. Considering patients who undergo an antibiotic regimen or cases of overuse of antibiotics, and the fact that we know little about how and for how long fly larvae accumulate these drugs in their system, how they eliminate them and how drugs affect their development (Soto 2008), we endeavored to investigate the effects of ampicillin on the postembryonic development of Chrysomya putoria (Wiedemann, 1830)
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