Possible Role of Selenium Nano-particles on Gentamicin-Induced Toxicity in Rat Testis: Morphological and Morphometric study

Abstract

Background: Gentamicin (GM) is abroad spectrum antibiotic that used worldwide. Exact mechanism of its testicular toxicity is still unknown. The present study aimed at studying the possible protective role of selenium nano-particles (SeNPs) on histological, biochemical, and morphometric changes in rat testis.Aim of Study: The current study was designed to elucidate the possible role of SeNPs on GM induced toxicity in rat testis.Materials and Methods: Twenty adult albino rats were divided into four groups, control, sham control, GM group, GM+ SeNPs group. The body weight (BW), testis weight, epididymis weight , seminal vesicle weight, prostate weight and serum testosterone level were determined. Testis sections were subjected to histological, morphometric, biochemical and statistical studiesResults: GM induced significant (sig) reduction in weight of testes, accessory sex organs, sperm count, motility and testosterone level. The drug also caused sig decrease in the activity of testicular catalase (CAT) and reduced glutathione (GSH) content, and sig increase in malondialdehyde (MDA) and H2O2 levels. Sig increase in basement membrane (BM) thickness, area % of caspase3 immunoexpression (IE) and in Ki67 count were recorded. Injection of SeNPs improved sig the previously mentioned changes.Conclusion: GM induced morphological, morphometric and biochemical changes on reproductive organs particularly testis of adult male rats. SeNPs administration with GM ameliorated these adverse effects that might be due to its antioxidant activity.