Abstract
Amiloride, an inhibitor of Na +/H + exchange, inhibited down-regulation of protein kinase C in HL60 cells induced by tumor-promoting phorbol ester in dose-dependent manner judging from immunoblot analysis. This inhibition was observed with regard to type I (γ), type II (β), and type III (α) isozymes of protein kinase C. On the other hand, monensin, a Na + ionophore, accelerated the down-regulation of protein kinase C induced by phorbol ester. When we examined 22Na + uptake by HL60 cells, the higher uptake was observed after stimulation with phorbol ester compared to the control cells and this 22Na + uptake was strongly inhibited by the addition of amiloride. However, monensin further stimulated the 22Na + uptake observed in phorbol ester-treated cells. These data suggest that the increase in intracellular Na + concentration may be one of the triggers for the induction of down regulation of protein kinase C.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
