Abstract
Low temperature (0 °C/1 hr) induced changes in the mastocytoma P815 cell surface morphology which mimicked those induced by alloimmune T-lymphocytes during the cytolytic process. These morphological changes consist of the formation and shedding of membrane vesicles (MV) from the P815 cell surface. When the low-temperature-induced MV shedding process takes place at 22 °C, it occurs without changes in membrane permeability to 51Cr, whereas at 37 °C, the kinetics of 51Cr release resemble those induced by alloimmune T lymphocytes. The effects of two microtubule-stabilizing agents, deuterium oxide and hexylene glycol, were investigated on the low-temperature-induced MV shedding process as well as on the lymphocyte-mediated cytolysis (LMC) of target cells. These agents were found to inhibit (a) the low-temperature-induced MV shedding, (b) the low-temperature-induced membrane permeability changes to 51Cr, and (c) the LMC process. These data indicate that disruption of microtubules may be involved in all these events.
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