Possible protective effect of quercetin against oxidative stress in liver from metabolic syndrome rats

Abstract

AbstractBackground/Aims:Metabolic syndrome (MS) is linked to type of type 2 diabetes mellitus associated with high glucose level and insulin resistance. Thioredoxin-1 (TRX-1) is localized in the cytoplasm and the mitochondria and controls cellular reactive oxygen species. The purpose of this study is to examine the relation between MS and oxidative stress, and effect of quercetin on oxidative stress via TRX-1 in liver of MS rats. Methods: Male wistar rats (200-250g in weight) were used. They were divided three groups. Control group, MS group receiving (935 mM sucrose in drinking water) and quercetin treated (15 mg/kg/day, administered by gavage) MS group. Protein level of TRX-1 was determined by Western blot.Results:Aspartate transaminase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH), levels increased in MS group as compared with the Con group. Total-antioxidant-status (TAS), superoxide-dismutase (SOD), and glutathione-peroxidase (GSH-Px) levels decreased in MS group when compared to Con group. Total-oxidant-status (TOS) levels increased in MS group as compared with the Con group. Triglycerides, total-cholesterol and LDL-cholesterol increased in MS group when compared with the Con group. TRX-1 level decreased in MS group and TRX-1 activity was lower in MS group than Con group. Conclusions: Treatment of quercetin decreased AST, ALT, LDH, and TOS levels while it increased GSH-Px, SOD, and TAS levels. Also, lipid profile changed with quercetin. In conclusion, treatment of quercetin significantly increased TRX-1 level and activity of TRX-1 in MS group. These data suggest that elevated oxidative stress in liver of MS may be reduced by quercetin.