Abstract
To clarify the involvement of botulinum ADP-ribosyltransferase sensitive low molecular G-proteins in 5-hydroxytryptamine (5-HT)-induced stimulation of phosphatidylinositol turnover, we examined the effects of 5-HT on inositol phosphates formation in COS 7 cells transfected with serotonin 2C subtype (5-HT 2c) receptor cDNA. 5-HT at 1 and 10 μM stimulated inositol phosphates formation in the cells transfected with 5-HT 2c receptor cDNA, but did not in non-transfected or vector-transfected cells. A typical 5-HT 2c receptor antagonist mianserin (0.3–3 μM) inhibited the 5-HT-induced inositol phosphates formation. Treatment with botulinum toxin D preparation (20 μg/ml, 8 h) that contained botulinum C3 ADP-ribosyltransferase, blocked the 5-HT-induced inositol phosphate formation, although botulinum toxin A preparation that did not contain the enzyme did not have an influence. These results support our previous findings suggesting that low molecular weight G-proteins ADP-ribosylated by botulinum ADP-ribosyltransferase are involved in phospholipase C activity.
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