Possible involvement of adenosine triphosphate in the mechanisms of protein sensibilization effect on the functional properties of the diaphragm and skeletal muscles

Abstract

Aim. To study the effect of adenosine triphosphate on the contractile function and non-quantum secretion of acetylcholine at the endplate zone (H-effect) of isolated mouse muscles on the background of protein sensibilization. Methods. The experiments were performed on white mice. Sensibilization was carried out by ovalbumin with an aluminum hydroxide gel. Mechanomyography studies were performed on isolated preparations of the diaphragm and of two leg muscles in isometric conditions. The contractions were recorded by a photoelectric converter. In order to study the condition of the postsynaptic membrane of the muscle fibers measured was the non-quantum secretion of acetylcholine. Compared were the parameters of muscle contraction before and after 5 min of perfusion with a solution of adenosine triphosphate. Results. In the diaphragm and in the soleus muscle the dynamics of the force vector of the muscular contraction correlated with the changes in the H-effect in all the studied experimental models. However, the extent of these changes in the sensibilized animals is less pronounced. Conclusion. It is possible that adenosine triphosphate affects the functional properties of both muscle during protein sensibilization; the change in the contraction force of the long extensor digitorum muscle during sensibilization is not related to the mechanisms of muscle excitation, mediated by adenosine triphosphate.