Abstract
The postnuclear supernatant and the crude lysosomal fraction were layered onto Ficoll gradients and centrifuged. After centrifugation ten fractions were ontained and each fraction was analyzed for protein, cytochrome oxidase, D-alanine oxidase and three to six acid hydrolases known as lysosomal enzymes. Latent and total activity were measured in the case of the acid hydrolases. Six marker lysosomal enzymes were distributed in broad patterns from the bottom to the top of the gradient on centrifugation of the postnuclear supernatant, though the markers of other particles were recovered in one or two fractions. After similar centrifugation of the crude lysosome fraction the ratios of the activity of three acid hydrolases differed, depending on their position on the gradient. These results indicate the presence of lysosomes heterogeneous in their content of acid hydrolases.
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