Possible effect of some dispersing agents (trypsin, EDTA) on the cell composition of Zajdela's hepatoma

Abstract

Prolonged (for 10 to 16 generations respectively) transplantation of Zajdela's ascites hepatoma with metastatic and ascites cells, coupled with repeated application of cell dispersing agents (trypsin and EDTA), led to the selective accumulation in the composition of both variants of the strain (“metastatic” and “ascites”) compared with cells characterized by a nucleus of smaller diameter than that of the ascites cells of the original strain. The distribution of the cell nuclei of the ascites cells of both variants of the strain by diameter was the same as in the cell population of a metastasis of the original strain in the paratracheal lymph gland. The study of the distribution of the cells of these populations among complexes of both variants of the strain, produced with the aid of treatment by dispersing agents, revealed a shift toward the numerical predominance of single tumor cells in the population. This shift was absent in the cell population of the metastasis of the original strain in the lymph gland.