Abstract
AimTo examine the potential of bovine follicular fluid (BFF) to attract bull spermatozoa.MethodsThe ability of the BFF to attract bull sperm was evaluated by observing changes in sperm migration after being placed in a cross‐column chamber. The movement parameters of the heads and flagella of the sperm that were attracted to the BFF were analyzed by using the Computer Assisted Sperm Analysis system.ResultsIt was observed that 61.6% of the bull sperm migrated toward the BFF when the BFF was used at a concentration of 0.1%, but 67.2% of the sperm did not migrate toward the BFF at a concentration of 10%. Relatively larger numbers of both precapacitated and postcapacitated bull sperm migrated toward the BFF (0.1%). The ability of the 0.1% BFF to attract sperm probably affected both the normal artificial insemination (AI) fertility sperm and the poor AI fertility spermatozoa. The flagellar curvilinear ratio of the sperm winding to the 0.1% BFF was significantly higher than that of the prewinding sperm.ConclusionThese results could suggest that BFF potentially attracts bull sperm at a certain concentration, irrespective of the capacitation status of the sperm. Although the mechanism by which this attraction occurs remains unclear, these data imply that it could be related to BFF‐dependent changes in the sperm flagellar curvilinear ratio.
Highlights
Sperm chemotaxis has been confirmed in species that have been fertilized in vitro.[1,2] Mammalian sperm migrate in the female genital tract and fertilize in the ampulla of the uterine tube
Based on a detailed kinetic analysis of migrated sperm in the female genital tract, it was found that chemotaxis,[1] thermotaxis,[6,7] and rheotaxis[8] are involved in sperm migration
The aim of this study was to evaluate the ability of bovine follicular fluid (BFF) to attract sperm by observing the changes that occur in the sperm’s migration and movement parameters in response to the BFF in a cross-c olumn chamber
Summary
Sperm chemotaxis has been confirmed in species that have been fertilized in vitro.[1,2] Mammalian sperm migrate in the female genital tract and fertilize in the ampulla of the uterine tube. Ascidian sperm swim in a straight line when they are exposed to a chemoattractant, and if the sperm should move away from the chemoattractant, they make sudden quick changes in direction to. | 134 turn back toward the chemoattractant.[2,12] Calcium bursts are induced by the concentration of the chemoattractant: sperm-activated and attracting factors triggers a sequence of flagellar responses that comprise quick turns in order to direct the sperm toward the eggs.[13] There have been few studies on the motility mechanism of mammalian sperm chemotaxis. The aim of this study was to evaluate the ability of bovine follicular fluid (BFF) to attract sperm by observing the changes that occur in the sperm’s migration and movement parameters in response to the BFF in a cross-c olumn chamber. Based on the obtained results, it is discussed whether bull spermatozoa have the potential to exhibit chemotaxis in response to the components of the BFF
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