Abstract
Modulating influence of electric impulses, visible light, some microelements is studied in parameters which are near to physio-logic ones on life activity of progenitor and mature cells of blood system in vitro and in situ. Content of granulocyte and fibroblastoid colony-forming units, concentration of colony-stimulating and colony-inhibiting activities, their concentration were determined , myelograms and morphologic signs of myelocariocytes apoptosis and necrosis were scored, saturation by chrom ions of peripheral blood erythrocytes was studied in mice bone marrow. The results showed that cell effects of physical-chemical influ-ences are small specific. Nevertheless, one may perform both positive and negative regulating influence on life activity of stromal precursors in dependence of energy density and stimulus concentration . The effect is due not only by direct but mediated reactions regulating direction and intensity of metabolic intracellular processes. It is suggested that one of target cells capable of change in-crease program of hemopoietic and stromal cells-progenitors of bone marrow in dependence of physical stimulus strength are lym-phocytes.
Highlights
Исследовано модулирующее влияние электрических импульсов, видимого света, некоторых микроэлементов в параметрах, приближенных к физиологическим, на жизнедеятельность родоначальных и зрелых клеток системы крови in vitro и in situ
Modulating influence of electric impulses, visible light, some microelements is studied in parameters which are near to physiologic ones on life activity of progenitor and mature cells of blood system in vitro and in situ
Content of granulocyte and fibroblastoid colony-forming units, concentration of colony-stimulating and colony-inhibiting activities, their concentration were determined, myelograms and morphologic signs of myelocariocytes apoptosis and necrosis were scored, saturation by chrom ions of peripheral blood erythrocytes was studied in mice bone marrow
Summary
Исследовано модулирующее влияние электрических импульсов, видимого света, некоторых микроэлементов в параметрах, приближенных к физиологическим, на жизнедеятельность родоначальных и зрелых клеток системы крови in vitro и in situ. С целью изучения воздействия физических факторов на костный мозг in situ бедренные кости 12 мышей Balb/c культивировали в 6 мл среды RPMI-1640 при температуре 37 С в течение 3 ч с АЭС ЖКТ, либо с АЭС ЖКТ с напылением хрома, либо с АФС, либо без таковых (контроль, точка 0). Взвесь эритроцитов периферической крови взрослого добровольца в количестве 25 106 клеток в 6 мл RPMI-1640 обрабатывали in vitro АЭС ЖКТ с напылением хрома в течение 10—120 мин. Проведенные исследования показали, что электрические импульсы способны оказывать как позитивное, так и негативное влияние на рост клеток in vitro. Число жизнеспособных клеток в асцитной опухоли Эрлиха in vitro через 24 ч после воздействия на клеточную взвесь электрическими импульсами и ионами хрома, X m
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