Possibilities of Laser-Doppler Flowmetry in assessment of functional state of microcirculation

Abstract

Aim . In the present study we evaluate the amplitude-frequency spectrum patterns of the relatively healthy individuals using the laser Doppler flowmetry with simultaneous registration of micro-flow in five points and application of orthostatic sample. Materials and methods . 144 healthy volunteers (69 women, 45 men) from 18 to 40 years old (the average age is 26±6) in a good physical condition were included in the exploration. The mobile flowmeters LAZMA-PF (Russia) was used to record blood flow. All patients were carried out by laser Doppler flowmetry with the use of orthostatic test. Measurements were recorded at the same time in five points (medial overhead areas, inner surface of forearms, overhead area). Results . We observed typical patterns of amplitude-frequency spectrum in the regions of arcs of aorta and upper hollow vein, as well as descending aorta and lower hollow vein. The changes are due to the body position alteration. By the monitoring blood flow in the upper extremity and the supercilium regions the spectral harmonic of the myogenic range (0.047–0.145Hz) dominated in the clinostatic position and the harmonic of the neurogenic range (0.02–0.046 Hz) was less noticed. In the signal spectrum obtained from the lower limbs the harmonic of the neurogenic range (0.02–0.046 Hz) dominated and the harmonic of the myogenic range (0.047–0.145Hz) was less noticed. In the orthostatic position in the region of upper extremities and supercilium, the harmonic amplitude of the neurogenic range increased regarding to the amplitude of the myogenic spectrum, and in the region of lower extremities, the harmonic amplitude of the myogenic range increased regarding to the amplitude of the neurogenic range. Conclusions . Using of the several sensors in the different body parts by the same time is improving the technique relevance. The healthy volunteers showed the typical patterns of slow wave part of ACS in the upper and lower parts of the body in clinostatic and orthostatic positions. The body position changing is reversing the patterns in the upper and lower body parts. Getting the postural test allows to assess the integrity of regulation mechanisms of microcirculation. The method used and the results need further investigation to determine the possibility to detect the functional and pathological changes, as well as compensatory possibilities of microcirculation in normal and in pathology.