Abstract
Inhibiting low-density lipoprotein (LDL) oxidation and cellular lipid accumulation to reduce foam cell formation plays a key role in preventing atherosclerosis. Casein hydrolysate (66% < 1 kDa) was separated into four different charged fractions. The inhibitory effect of peptide fractions on LDL oxidation and cellular lipid accumulation was analysed using a CuSO4 cell-free system and a Cu2+-mediated and ox-LDL-induced Raw264.7 macrophage cell-based system. Casein peptide fractions not only significantly inhibited LDL oxidation but also prevented cellular lipid accumulation. Positively charged fractions exhibited stronger inhibitory effects than negatively charged fractions. Seven peptides with different charge properties were synthesised. With the increase in net positive charge, the ability of peptides to inhibit LDL oxidation was enhanced. Peptides containing lysine presented better inhibition than those that contained histidine. This study suggests that casein hydrolysate, especially positively charged peptide fractions, could be used as a natural antioxidant in functional foods to prevent atherosclerosis.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
