Abstract
A positive selection plasmid vector (pLA7), containing a unique BclI site, and its use in the facile selection of a library of overlapping DNA fragments, generated by a partial digest with Sau3A, is described. Selection depends on the 5-fluorouracil + 5′AMP resistance of upp− ush− [pLA7ush:: cloned DNA] cells, whereas upp−ush− [pLA7ush +] cells are sensitive under the same conditions.
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