Positive Effect of Injectable Platelet Rich Fibrin (i-PRF) on Vital Bone Formation in Graft Reconstruction of the Mandible: A Histologic and Histomorphometric Study

Abstract

In this clinical case series, relative centrifugation force (RCF) and centrifugation time (CT) were reduced to produce the liquid form of platelet rich fibrin (i-PRF). The goal of this study is to histologically evaluate if the liquid form of PRF has a positive effect on vital bone formation in the grafted mandible using mineralized freeze-dried allogeneic bone. Materials and Methods: Forty-two patients completed immediate bone grafting after a non-restorable endodontically treated molar tooth was extracted in preparation for future implant surgery. Each bone graft was augmented with i-PRF. To process i-PRF, relative centrifugation force (RCF) was reduced from 2,700 RPM to 700 RPM (60g). Centrifugation time was reduced from 12 minutes to 3 minutes. After an average bone graft healing time of 8 to 12 weeks, bone core samples were obtained at the time of implant surgery for histological and histomorphometric analysis. Results: Forty-two bone cores were harvested at the time of implant surgery for histological and histomorphometric analysis. The liquid form of PRF resulted in greater percentages of vital bone formation (average 87.4 %) compared to the existing published literature. Conclusion: i-PRF resulted in greater percentages of new vital bone formation compared to the published literature. This could be due to the continuous slow release and delivery of growth factors in i-PRF. Further clinical use of iPRF is needed to evaluate the full potential of bone regeneration.