Positive correlation between pregnancy-associated plasma protein-A level and OX40 ligand expression in patients with acute coronary syndromes

Abstract

Objective Increasing evidence show that serum pregnancy-associated plasma protein-A (PAPP-A) and OX40 ligand (OX40L) expression have been implicated in acute coronary syndromes (ACS). We investigated the relationship between PAPP-A level and OX40L in serum and membrane-bound OX40L in patients with ACS. Methods The present study included normal controls ( n = 30), patients with stable angina (SA) ( n = 60) and patients with ACS, including unstable angina (UA) ( n = 50) and acute myocardial infarction (AMI) ( n = 30). Serum concentrations of PAPP-A and soluble OX40L (sOX40L) were determined with Elisa, whereas the expression of OX40L on monocytes were analyzed with flow cytometry. Results The expression of OX40L in peripheral monocytes in patients with UA [25.6 ± 5.5 mean fluorescence intensity (MFI)] and AMI (29.4 ± 6.3 MFI) were significantly higher than those in patients with SA (10.6 ± 2.8 MFI) and controls (11.1 ± 3.5 MFI). Both sOX40L and PAPP-A in patients with UA (15.7 ± 4.9 ng/mL, 25.4 ± 6.8 μg/mL, respectively) and AMI (17.1 ± 5.3 ng/mL, 26.3 ± 5.6 μg/mL, respectively) were significantly higher than those in patients with SA (3.4 ± 1.4 ng/mL, 9.6 ± 2.1 μg/mL, respectively) and controls (3.9 ± 1.3 ng/mL, 8.5 ± 2.8 μg/mL, respectively) ( P < 0.001). Interestingly, a positive correlation was found between sOX40L, membrane-bound OX40L and serum PAPP-A levels (r 1 = 0.54, r 2 = 0.51; P < 0.0001). Both serum and membrane-bound OX40L and PAPP-A levels significantly correlated with complex coronary stenosis (r 1 = 0.56, r 2 = 0.55, r 3 = 0.40; P < 0.001). Conclusion PAPP-A level was significantly related to soluble and membrane-bound OX40L in patients with ACS. Enhanced level of serum PAPP-A and sOX40L might represent a prognostic marker for coronary disease activity.