Abstract
Partial reductive hydrolysis was used to produce oligosaccharide alditols from repetitive sulfated galactans obtained from four Rhodophyta species: κ-carrageenan (from Kappaphycus alvarezii), θ-carrageenan ( Gigartina skottsbergii—alkali-treated λ-carrageenan), agarose 6-sulfate ( Gracilaria domingensis), and pyruvylated agarose 2-sulfate ( Acanthophora spicifera—alkali-treated pyruvylated agaran sulfate). Each hydrolyzate was submitted to anion-exchange and gel-filtration chromatography, and the isolated oligosaccharide alditols were identified by 1D and 2D NMR spectroscopy and by ESI mass spectrometry. The positional isomers of the sulfated oligosaccharide alditols were then completely resolved by capillary electrophoresis in a borate buffer. Attempts to correlate the availability of the hydroxyl groups for borate complexation with the relative migration of the oligosaccharides are presented.
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