Abstract

Recent experiments by Ogawa, Tomizawa & Fuke (1968) have shown that it is possible to isolate intracellular λ DNA having a circular structure but with an extra length of DNA extending between two branch points. An attempt has been made to map the position of these branch points with respect to the point on the circular structure corresponding to the ends of the mature λ phage DNA molecule. If the circular branched molecules do, in fact, represent replicating DNA, then certain conclusions may be drawn concerning the starting point and direction of replication.

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