POS1109 GENETIC MARKERS PREDICT THE DEVELOPMENT OF POSTOPERATIVE PAIN

Abstract

BackgroundPostoperative pain (POP) is a serious complication that affects the outcome of total arthroplasty (TA) of the knee (CJ) and hip (HJ) joints in patients with osteoarthritis (OA). The search for the genetic characteristics of POP is an urgent direction in the study of this problem.ObjectivesTo determine the relationship between the polymorphisms of the KCNS1, COMT, and OPRM1 genes and the development of postoperative pain in patients with osteoarthritis of the knee joint and hip joint who underwent total arthroplasty.MethodsThe study group consisted of 95 patients with knee osteoarthritis and / or hip joint osteoarthritis (64.6% of women; mean age - 65.4 ± 9.0 years) who underwent TA CJ (47.8%) or TA HJ (52.2 %). The presence of POP was determined when it persisted or appeared after 3 and 6 months. After surgery, pain in the area of the operated joint ≥40 mm by 100 mm visual analogue scale. All patients underwent genotyping of KCNS1 (rs734784), COMT (rs6269, rs4633), and OPRM1 (rs1799971) gene polymorphisms by real-time polymerase chain reaction using original sequence-specific primers and probes labeled with various fluorescent labels. Registration and interpretation of the obtained results were carried out on a DT-96 amplifier (DNA-Technology LLC, Russia).ResultsPOP was observed in 32.6% of patients who underwent TA CJ or TA HJ. The incidence of POP after TA CJ and TA HJ was 30.2% and 34.0%, respectively (p = 0.882). There were no differences in the frequencies of genotypes of the studied genes (p>0.05). The presence of the homozygous GG genotype of the KCNS1 gene polymorphism (rs734784) was associated with the presence of POP in accordance with the recessive genetic model (GG vs AA + AG; odds ratio (OR) - 3.96 [95% confidence interval (CI): 1, 51; 10.37]; p = 0.005). The presence in the genotype of the mutant allele T (TT + CT) of the COMT polymorphism (rs4633) reduced the risk of developing POP compared with the carriage of the CC genotype (OR = 0.32 [95% CI: 0.12; 0.83]; p = 0, 02) according to the dominant genetic model. There was no statistically significant correlation between the development of POP and the carriage of various genotypes and alleles of the COMT (rs6269) and OPRM1 (rs1799971) genes.ConclusionThere is a statistically significant association between the polymorphism of the KCNS1 (rs734784) and COMT (rs4633) genes and the development of chronic POP in patients who underwent TA CJ and TA HJ. Further studies of the genetic predisposition to POP are required using more clinical material.Disclosure of InterestsNone declared