Portable Six-Channel Laser Speckle System for Simultaneous Cerebral Blood Flow and Volume Measurement with Potential Application for Characterization of Brain Injury.

Abstract

In regional cerebrovascular monitoring, cerebral blood flow (CBF) and cerebral blood volume (CBV) are key metrics. Simultaneous, non-invasive measurement of CBF and CBV at different brain locations would advance cerebrovascular monitoring and pave the way for brain injury detection, as current brain injury diagnostic methods are often constrained by high costs, limited sensitivity, and reliance on subjective symptom reporting. This study's aim is to develop a multi-channel non-invasive optical system for measuring CBF and CBV at different regions of the brain simultaneously with a cost-effective, reliable, and scalable system capable of detecting potential differences in CBF and CBV across different regions of the brain. The system is based on speckle contrast optical spectroscopy (SCOS) and consists of laser diodes and board cameras which have been both tested and investigated for safe use on the human head. Results on a cohort of five healthy subjects indicated that the dynamics of both CBF and CBV were synchronized and exhibited similar cardiac period waveforms across all six channels. As a preliminary investigation, we also explored the potential use of our six-channel system for detecting the physiological sequela of brain injury, involving a subject with significant structural brain damage compared to another with lesser structural brain damage. The six-point CBF and CBV measurements were compared to MRI scans, revealing that regions with altered blood dynamics closely correlated with the injury sites identified by MRI.