Abstract
Dexamethasone (DEX) is widely used because of its anti-inflammatory, anti-endotoxin, anti-shock, and stress-enhancing response activities. It can increase the risk of diabetes and hypertension if it is abused or used improperly. However, there is a lack of sensitive and rapid screening methods for DEX in food. In this study, a time-resolved fluorescent microspheres immunochromatographic assay (TRFM-ICA) integrated with a portable fluorescence reader was developed for the quantitative detection of DEX in milk and pork. The cut-off values of the TRFM-ICA were 0.25 ng/mL and 0.7 µg/kg, respectively. The limits of quantitation (LOQs) were 0.003 ng/mL and 0.062 µg/kg, respectively. The recovery rates were 80.0–106.7%, and 78.6–83.6%, respectively, with the coefficients of variation ranging 6.3–12.5%, and 7.5–10.3%, respectively. A parallel experiment for 20 milk and 10 pork samples with LC-MS/MS was carried out to confirm the performance of the on-site application of the developed TRFM-ICA. The results of the two methods are basically the same; the correlation (R2) was >0.98. The establishment of TRFM-ICA will provide a new sensitive and efficient technical support for the rapid screening of DEX in food.
Highlights
(c) The surface of time-resolved fluorescent microspheres (TRFM) is modified with carboxyl or other functional groups, which are used for covalent coupling with proteins or Ab, improving the stability of the conjugates
The spiked milk and pork samples with a series of DEX concentrations were used to assess the sensitivity of the developed TRFM-ICG; each spiked concentration was detected in triplicate
The precision and accuracy of the TRFM-ICG were assessed by the coefficient of variation (CV) and recovery, respectively
Summary
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Effects, and trace detection requirements of DEX, it is imperative to establish a rapid and sensitive detection method for DEX in food to ensure the health of humans and animals. In order to provide a stable, sensitive, reliable, and rapid detection method for DEX residue detection, ICG based on time-resolved fluorescent microspheres (TRFM). (c) The surface of TRFM is modified with carboxyl or other functional groups, which are used for covalent coupling with proteins or Ab, improving the stability of the conjugates These features are ideal for the development of ICG. A portable, compact desk reader was used to quantify results This integrated strategy could provide valuable technical support for the on-site detection of DEX in animal-derived food
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