Portable Microfluidic Biosensing System for Real-Time Analysis of Microdialysate in Transplant Kidneys.

Isabelle C Samper,Tonghathai Phairatana,Sally A N Gowers,Bynvant Sandhu,Natalie Vallant,Martyn G Boutelle,Marsilea A Booth,Vassilios Papalois,Chu Wang,Thomas Watts

doi:10.1021/acs.analchem.9b03774

Isabelle C Samper, Tonghathai Phairatana + Show 8 more

Open Access

https://doi.org/10.1021/acs.analchem.9b03774

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Abstract

Currently, there is a severe shortage of donor kidneys that are fit for transplantation, due in part to a lack of adequate viability assessment tools for transplant organs. This work presents the integration of a novel wireless two-channel amperometric potentiostat with microneedle-based glucose and lactate biosensors housed in a 3D printed chip to create a microfluidic biosensing system that is genuinely portable. The wireless potentiostat transmits data via Bluetooth to an Android app running on a tablet. The whole miniaturized system is fully enclosed and can be integrated with microdialysis to allow continuous monitoring of tissue metabolite levels in real time. We have also developed a wireless portable automated calibration platform so that biosensors can be calibrated away from the laboratory and in transit. As a proof of concept, we have demonstrated the use of this portable analysis system to monitor porcine kidneys for the first time from organ retrieval, through warm ischemia, transportation on ice, right through to cold preservation and reperfusion. The portable system is robust and reliable in the challenging conditions of the abattoir and during kidney transportation and can detect clear physiological changes in the organ associated with clinical interventions.

Highlights

Kidney transplantation is the optimal treatment for patients with end-stage kidney disease
DCD organs tend to be under-utilized as there is a period of prolonged warm ischemia that can cause the organ not to function in the initial period following transplantation.[2−4] expanded criteria donors (ECD) organs, such as those from donors who are aged 60 years and older or who do not meet defined criteria,[5] are at a higher risk of graft failure;[5] these transplants have been shown to be beneficial compared with the alternative of chronic dialysis treatment.[6]
We describe the development of a miniaturized wireless portable analysis system for continuous measurement of dialysate glucose and lactate levels in real time

Summary

Introduction

Kidney transplantation is the optimal treatment for patients with end-stage kidney disease. We describe the development of a miniaturized wireless portable analysis system for continuous measurement of dialysate glucose and lactate levels in real time.

Results

Conclusion