Portable Closed Loop Feedback System for Control of the Blood Glucose Level in the Pig

Abstract

To establish the difference in glucose consumption of healthy compared to diabetic pigs, a transportable system for the feedback control of insulin and glucose was used in conscious pigs. The system for controlling glucose levels, as well as glucose consumption, was easy to use and adjusted smoothly to rapid changes in insulin level. Five days prior to the first day of experiment, healthy animals were prepared with intravenous jugular catheters tunnelled subcutaneously to the neck. On the first day of experiment, measurements of glucose were continuously taken before and after food ingestion. The animals were rendered diabetic after 2 days using Alloxan and streptozotocin. The measurements were then repeated. To be able to obtain constant glucose levels of approximately 3 mmol during both fasting and postprandial periods requires only small glucose adjustment infusion in the diabetic pigs, as opposed to healthy ones. Using the described system, it would be possible to evaluate subclinical diabetes and control unstable diabetics with different therapeutic regimes.