Abstract
BackgroundPorphyromonas gingivalis is strongly associated with the development, progression, severity and recurrence of periodontitis. Periodontal ligament stem cells (PDLSCs) play an important role in the maintenance of periodontal tissue self-renewal and repair. The purpose of this study was to investigate the ability of P. gingivalis to infect PDLSCs using an in vitro monolayer model.MethodsWe separated and cultured primary PDLSCs using the tissue block with limiting dilution method. The efficiency of P. gingivalis (ATCC 33277) infection of PDLSCs was measured using agar plate culture and quantitative polymerase chain reaction (q-PCR) methods. PDLSCs infected with P. gingivalis were also observed by transmission electron microscopy.ResultsWe assessed stem cell properties including cell morphology, clone formation, growth activity, cell surface antigens and multiple differentiation capacity. The infection rates of P. gingivalis in PDLSC at MOIs of 50, 100, 200, and 500 were 5.83%, 8.12%, 7.77% and 7.53% according to the agar plate culture method. By q-PCR, the efficiencies of P. gingivalis infection of PDLSCs at MOIs of 50, 100, 200, and 500 were 6.74%, 10.56%, 10.36% and 9.78%, respectively. Overall, the infection efficiency based on q-PCR was higher than that according to agar plate culture. Using transmission electron microscopy, we verified that P. gingivalis (ATCC 33277) could infect and invade PDLSCs after 2 h of incubation, and endocytic vacuoles were not found surrounding the internalized bacteria.ConclusionsIn conclusion, our data demonstrate that P. gingivalis can invade PDLSCs.
Highlights
Porphyromonas gingivalis is strongly associated with the development, progression, severity and recurrence of periodontitis
Large numbers of bacteria reside in the oral cavity, including Porphyromonas gingivalis, a gram-negative anaerobic bacterium
Many studies have shown that periodontal ligament cells comprise a heterogeneous group that includes osteoblasts, fibroblasts and stem cells, among others
Summary
Porphyromonas gingivalis is strongly associated with the development, progression, severity and recurrence of periodontitis. Periodontal ligament stem cells (PDLSCs) play an important role in the maintenance of periodontal tissue self-renewal and repair. Periodontitis, a chronic infectious disease in periodontal support tissues that can induce or aggravate multi-system diseases such as diabetes [1], cardiovascular disease [2] and chronic obstructive pulmonary disease [3], has become one of the most common human diseases causing damage to both oral and general health. The virulence factors of P. gingivalis, periodontitis can be controlled through conventional therapies, it is difficult to restore damaged periodontal structures [8]. During infection, bacteria can alter the surrounding environment and inhibit endogenous cell differentiation [9]. Chronic inflammation can inhibit tissue repair by reducing cell
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
