Abstract
This paper investigates the possibility of utilizing tin-oxide (SnO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> )-Coated regenerated cellulose as a disposable and low-cost alternative transducer for urea detection. A thin layer of SnO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> is coated on regenerated cellulose films via liquid-phase deposition technique. Cellulose-SnO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> hybrid nanocomposite urea biosensor is prepared by immobilizing urease on the SnO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> layers by physical absorption method. The resulting biosensor shows a linear response up to 42 mM. Also it is found that the proposed sensor exhibits the same level of sensitivity for seven days; in other words, the sensitivity of the sensor did not degrade with time and remained same for 7 days. This urea sensor is inexpensive, flexible, and disposable.
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