Abstract
Spherical and macroreticular epithiopropyl methacrylate-ethylene glycol dimethacrylate (thiiranyl) and glycidyl methacrylate-ethylene glycol dimethacrylate (glycidyl) copolymer beads of two differing copolymer compositions were synthesized. Particle size was estimated to be in the range 250–420 μm for both sets of copolymers. The anomalous increase in both surface area and pore volume with crosslinking density reflects a bimodal distribution of the internal porous structure in both these copolymers. These reactive copolymer beads were investigated for the binding and expression of a biocatalyst, penicillin G acylase. The activity of immobilized penicillin G acylase obtained, 191 IU/g, compares well with established industrial immobilized enzymes.
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