Abstract
A novel porous silicon based optical Fabry-Perot cavity structure is prepared as a label-free immunosensor platform for detecting antigen-antibody. The lagurus zona pelluciad 3 (LZP3) and the specificity of the polyclonal anti-LZP3 antibodies are employed in our laboratory as the target and the probe, respectively. Firstly, the antibodies are immobilized to the porous silicon optical cavity using silanization and glutaraldehyde (GA) chemistry. And then, after the antigen-antibody reaction, it is monitored that the red shift of the reflection spectrum of the immunosensor increases with the antigen concentration. This research also plays a potential role for the extensive applications in immunoassay.
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