Porous Poly(Hexamethylene Biguanide) Hydrochloride Loaded Silk Fibroin Sponges with Antibacterial Function.

Ahui Liang,Min Zhang,Mingzhong Li,Longxing Niu,Hong Luo,Yanfei Feng

doi:10.3390/ma13020285

Abstract

In order to endue silk fibroin (SF) sponges with antibacterial function, positively charged poly(hexamethylene biguanide) hydrochloride (PHMB) was incorporated in SF through electrostatic interaction and by freeze-drying technique. The influence of PHMB on the structure and antibacterial activities of SF sponges was investigated. The zeta potential of SF was increased significantly when PHMB was incorporated in SF. The pores with size from 80 to 300 µm and the microscale holes in the pore walls within PHMB-loaded SF sponges provided the channels of PHMB release. The PHMB loaded in the porous sponges showed continuous and slow release for up to 20 days. Effective growth inhibition of both Escherichia coli and Staphylococcus aureus was achieved when the mass ratio of PHMB/SF was higher than 2/100. These results suggest that the porous PHMB/SF sponges have the potential to be used as a novel wound dressing for open skin wounds.

Highlights

As temporary wound coverings, wound dressings function as a skin barrier
The zeta potential of the complexes increased to +2.70 ± 0.26 mV when the mass ratio of poly(hexamethylene biguanide) hydrochloride (PHMB)/silk fibroin (SF) was 10/100, which showed that the surface of complexes had a large positive charge
Complexes increased significantly when PHMB was incorporated in SF protein

Summary

Introduction

Wound dressings function as a skin barrier. Wound dressings can protect the wound from invasion by external microorganisms and prevent the evaporation and loss of water and body fluid from the wound, providing a favorable environment for wound healing [1]. Skin defects are always accompanied by bacterial infection [2,3]. The necrotic tissue rich in denaturing proteins and moist environment created by wound exudate provide favorable conditions for bacterial growth and reproduction. If bacterial infection is not effectively controlled, the continuous production of bacterial endotoxins and inflammatory factors that stimulate the production of various cells will seriously hinder the healing of the wound, affect the growth rate of the epidermis, or even cause multiple organ failure, eventually threatening the life of patients [4]. Inhibiting the growth of bacteria at the wound is the key to wound healing [5,6,7]

Objectives

Methods

Results

Discussion

Conclusion