Abstract

Localization of lipidomes and tracking their spatial changes by mass spectrometry imaging (MSI) is critical for the mechanism studies on living process, disease, and therapeutic treatment. However, due to the strong ion suppression in complex biotissue, the imaging coverage for lipids with low polarity or low abundances, such as glycerolipids and sphingolipids, is usually limited. To address this issue, we utilized a porous graphitic carbon (PGC) material to imprint brain tissue sections for selective enrichment of neutral lipids with polar phospholipids removed. Then, the tissue imprint was scanned for desorption by the ambient liquid extraction MSI system. It was found that on the PGC surface, hydrophobic interaction dominates in protic solvents, and polar interaction dominates in aprotic solvents. Accordingly, methanol was selected as the spray solvent for tissue imprinting, and 75% acetonitrile-methanol was selected as the desorption solvent for the ambient liquid extraction MSI system. The results showed that glycerides had high recoveries after the imprinting-desorption process (recovery ∼ 70%) with phospholipids eliminated (recovery < 7%). To increase the transferring efficiencies of lipids from tissue onto PGC, electrospray was used for solvent application during imprinting, and the signals of diglycerides (DGs) in the imprint MSI of brain tissue increased by 2-3 times as compared to those via air spray. Finally, the new imprint MSI approach was applied to the imaging of the rat cerebellum and was compared with direct tissue MSI. The results showed that with imprint MSI, the coverage of DGs, sphingomyelins (SMs), and ceramides was enhanced by 4-5-fold (32 vs 6, 4 vs 1, and 5 vs 0). The ion images showed that with imprint MSI, higher signal intensities and clearer spatial distribution of DGs and SMs were obtained without interference from phosphatidylcholine signals compared with tissue MSI. This new method provides a complementary approach for traditional MSI to address the issues in imaging poorly ionizable or low-abundance lipids.

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