Abstract
The effect of porous alpha-tricalcium phosphate (α-TCP) with immobilized basic fibroblast growth factor (bFGF) on bone regeneration was evaluated in a canine mandibular bone defect model. Identical bone defects were made in the canine mandible; six defects in each animal were filled with porous α-TCP with bFGF bound via heparin (bFGF group), whereas the other was filled with unmodified porous α-TCP (control group). Micro-computed tomography and histological evaluation were performed two, four and eight weeks after implantation. The bone mineral density of the bFGF group was higher than that of the control group at each time point (p < 0.05), and the bone mineral content of the bFGF group was higher than that of the control group at four and eight weeks (p < 0.05). Histological evaluation two weeks after implantation revealed that the porous α-TCP had degraded and bone had formed on the surface of α-TCP particles in the bFGF group. At eight weeks, continuous cortical bone with a Haversian structure covered the top of bone defects in the bFGF group. These findings demonstrate that porous α-TCP with immobilized bFGF can promote bone regeneration.
Highlights
Bone defects attributed to severe periodontitis, trauma, and injury are frequently encountered in the fields of oral implant and orthopedics [1,2]
Tricalcium phosphate (TCP) is a bioresorbable bone substitute used for oral implants and in orthopedics
Α-TCP was implanted into a canine mandibular bone defect and bone formation and remodeling wereevaluated evaluatedfor forup uptotoeight eightweeks
Summary
Bone defects attributed to severe periodontitis, trauma, and injury are frequently encountered in the fields of oral implant and orthopedics [1,2]. Human recombinant bone morphogenetic protein 2 belongs to the transforming growth factor-beta (TGF-β) superfamily of proteins [8]. Human bone morphogenetic protein 2 belongs to the transforming growth factorbeta (TGF-β) superfamily of proteins [8] It is a key molecule in bone metabolism owing to its ability to induce differentiation mesenchymal cells intoosteoblasts osteoblasts[8]. A carrier/delivery system is necessary for the binding and controlled release release of growth factors of growth factors such as such bFGF.as bFGF In this this study, study,we wesuccessfully successfullyimmobilized immobilized porous α-TCP heparin evaluated. In onon porous α-TCP viavia heparin andand evaluated the the effect of this carrier on bone regeneration in a canine mandibular bone defect model. Effect of this bFGF carrier on bone regeneration in a canine mandibular bone defect model
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