Abstract
A resource consisting of one million porcine ESTs is described, providing an essential resource for annotation, comparative genomics, assembly of the pig genome sequence, and further porcine transcription studies.
Highlights
Knowledge of the structure of gene expression is essential for mammalian transcriptomics research
expressed sequence tags (ESTs) sequences and cDNA libraries The analyses presented here are based on 1,021,891 porcine EST sequences, of which 636,516 were extracted from the Sino-Danish (SD) resource and 385,375 from GenBank [30]
The SD EST sequences were generated from 98 cDNA libraries covering 35 tissues listed in Table 1 (97 non-normalized and 1 normalized [Pla])
Summary
Knowledge of the structure of gene expression is essential for mammalian transcriptomics research. The porcine genome has been characterized intensively through development of linkage maps, comparative maps, and physical maps [1,2] and Humphray and co-workers (unpublished data) These studies highlight the importance of genome research in pigs. We recently showed that the evolutionary distance between the porcine and human genome sequences is smaller than the distance between mouse and human [3] This provides a rationale for use of porcine sequences in gene expression comparisons with human and in transcriptome analysis of multiple tissues and organs [4,5] because, in contrast to human, there is easy access to tissues from the pig, including tissues from various embryonic developmental stages. It is possible to search for genes that are expressed in a wide range of tissues, including genes that are of importance to embryonic development, because 24 of the 98 libraries used in the study are from various developmental stages
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