Abstract

Porcine stress syndrome (PSS) is one kind of molecular genetics defect which will cause malignant hyperthermia syndrome in pigs. It was reported that mutation of pig rynodine receptor (RYR1) gene is the main reason for PSS. The aim of this study was to test the RYR1 genotype of 10 Mangalitsa pigs using a polymerase chain reaction-restriction endonuclease fragment length polymorphism (PCR-RFLP) technique, which is a reliable and simple method for RYR1 gene testing. Extraction of DNA was done by using hair follicles. The results showed that the RYR1 genotype of all the 10 porcine cases were negative. These results suggested that Mangalitsa pig could be one of the porcine breeds selectively bred for medical and clinically experiments.

Highlights

  • Meat is one of the main sources of protein in the human diet and pork is one of the most produced and consumed worldwide

  • It was reported that mutation of pig rynodine receptor (RYR1) gene is the main reason for Porcine stress syndrome (PSS)

  • The results showed that the RYR1 genotype of all the 10 porcine cases were negative

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Summary

Introduction

Meat is one of the main sources of protein in the human diet and pork is one of the most produced and consumed worldwide. Breeding of domesticated animals for food production historically was directed to increase productive performances. This way highly selected, meat-type pig breeds were developed. Lundstrom et al (1995) showed that the PSS gene affected the quality of the M. longissimus dorsi and resulted in less tender, less desirable pork. This condition causes the carcasses to be classified as unacceptable. The aim of this study was to determine the presence of PSS gene in Mangalitsa, a Serbian autochthonous pig breed. Extraction of DNA was done from a hair root that can be a valuable source of DNA for genotyping

Materials and Methods
PCR-RFLP
Results
Conclusion
Full Text
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