Abstract
BackgroundPorcine epidemic diarrhea virus (PEDV) is an important pathogen in swine and is responsible for substantial economic losses. Previous studies suggest that the PEDV E protein plays an important role in the viral assembly process. However, the subcellular localization and other functions of PEDV E protein still require more research.MethodsThe subcellular localization and function of PEDV E protein were investigated by examining its effects on cell growth, cell cycle progression, interleukin-8 (IL-8) expression and cell survival.ResultsThe results show that plenty of PEDV E protein is localized in the ER, with small quantities localized in the nucleus. The PEDV E protein has no effect on the intestinal epithelial cells (IEC) growth, cell cycle and cyclin A expression. The cells expressing PEDV E protein express higher levels of IL-8 than control cells. Further studies show that PEDV E protein induced endoplasmic reticulum (ER) stress and activated NF-κB which is responsible for the up-regulation of IL-8 and Bcl-2 expression.ConclusionsThis study shows that the PEDV E protein is localized in the ER and the nucleus and it can cause ER stress. The PEDV E protein had no effect on the IEC growth and cell cycle. In addition, the PEDV E protein is able to up-regulate IL-8 and Bcl-2 expression.
Highlights
Porcine epidemic diarrhea virus (PEDV) is an important pathogen in swine and is responsible for substantial economic losses
Construction of a recombinant plasmid pEGFP-N1-E and PEDV E protein expression The Western blot assay (Figure 1A) showed that the cells transfected with the pEGFP-N1-E plasmid and pEGFP-N1 plasmid expressed approximate molecular mass of 35 kDa and 27 kDa proteins that were detected by anti-GFP monoclonal antibodies
The Western blot assay (Figure 1B) showed that the cells transfected with the pEGFP-N1-E plasmid expressed an approximate molecular mass of 35 kDa protein that were detected by anti- PEDV polyclonal antibodies
Summary
Porcine epidemic diarrhea virus (PEDV) is an important pathogen in swine and is responsible for substantial economic losses. Previous studies suggest that the PEDV E protein plays an important role in the viral assembly process. Porcine epidemic diarrhea (PED) is an acute and highly contagious enteric disease of swine characterized by severe enteritis, vomiting, and watery diarrhea and results in high mortality in piglets [1]. A recombinant MHV virus was constructed with the E gene deleted. No data were reported about the subcellular localization of PEDV E protein, its effects on cell growth and cell cycle progression. This study initiates the subcellular localization of PEDV E protein and elucidates the effects and mechanisms of this protein on cell growth and cell cycle
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