Abstract
Porcine circovirus-like virus P1 is an important pathogen of the current pig industry, the infection mechanism is not entirely clear. Wnt signaling pathway plays an important role in the growth of young animals and infection of some viruses. This study was designed to demonstrate the effects of P1 infection on the Wnt signaling pathway. In vivo experiments, we demonstrated the down-regulatory effects of P1 infection in piglets and mice on the downstream components expression levels of Wnt signaling pathway, and the effects of Wnt signaling pathway activation on the pathogenesis of P1. In vitro studies, we found P1 infection down-regulated protein level of β-catenin and mRNA level of mmp2, prevented the β-catenin from entering into nucleus, abolished the TCF/LEF promoter activity, proved that P1 could inhibit the activation of Wnt signaling pathway in vitro. Finally, we found that VP1 of P1 virus also had the inhibitory effects on Wnt signaling pathway in vitro, elucidated the mechanism of P1’s inhibitory effects on the Wnt signaling pathway and offered the possibility that the suppression of Wnt signaling pathway was involved in the post-weaning multisystemic wasting syndrome (PMWS), laying a foundation for elucidating the pathogenesis of P1.
Highlights
Porcine circovirus like virus is one of the recently discovered minimal genomic DNA viruses (648 nucleotides)
The discovery of the virus occurred on the diagnosis of porcine circovirus type 2 (PCV2) in the tissue sample of post-weaning multisystemic wasting syndrome (PMWS)
The relative average weight gain in challenge group was 0.03859 ± 0.00479 kg, while average daily weight gains (ADWG) was 0.06004 ± 0.00970 kg in control group, indicating that weight losses were significantly decreased in challenge group compared to control group
Summary
Porcine circovirus like virus is one of the recently discovered minimal genomic DNA viruses (648 nucleotides). The discovery of the virus occurred on the diagnosis of porcine circovirus type 2 (PCV2) in the tissue sample of post-weaning multisystemic wasting syndrome (PMWS). The porcine circovirus-like virus shares similar sequence with the PCV2 ORF2 (opening read frame 2) sequence, named porcine circovirus-like virus P1 and P2 (Wen et al, 2011). Wen successfully constructed P1, P2 single-copy molecular cloning and double-copy tandem molecular cloning, after transfection into PK-15 cells, inclusion bodies were found in the cytoplasm and nucleus. During continuous passage of five generations, P1 and P2 agents can be detected by PCR in the cells transfected with P1, P2 double-copy tandem molecular cloning (Wen et al, 2012a,b,c).
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