Abstract
Porcine circovirus type 2 (PCV2) is an emergent single-stranded DNA virus found all over the world in domestic pigs and wild boars that causes infectious disease with a great impact on swine productivity. PCV2 has 1.7 kb genome that includes two main genes, which encode replication-related protein (rep) and the major structural capsid (cap) protein. Both of these genes can be used as target sequences for the primer design for the detection of PCV2 as well as for sequencing of designated regions. We carried out a screening due to the PCV2 circulating among the wild boar population in 10 regions of Ukraine. PCR screening was performed using primer pairs designed on the target sequences of the replicative and capsid genes. According to the results of the research, the presence of genetic material of PCV2 was found in 31.8% of the tested samples. The developed set of primers may be suitable for diagnostics, as well as for the development of specific sites for the purpose of sequencing of PCV2 cap-gene due to the obtained DNA samples during epizootic screening
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal for Veterinary Medicine, Biotechnology and Biosafety
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
