Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture.

Girdhari Rijal,Weimin Li,Jing Wang,Ilhan Yu,Roland Chen,David Gang

doi:10.3390/ijms19102912

Abstract

Porcine mammary fatty tissues represent an abundant source of natural biomaterial for generation of breast-specific extracellular matrix (ECM). Here we report the extraction of total ECM proteins from pig breast fatty tissues, the fabrication of hydrogel and porous scaffolds from the extracted ECM proteins, the structural properties of the scaffolds (tissue matrix scaffold, TMS), and the applications of the hydrogel in human mammary epithelial cell spatial cultures for cell surface receptor expression, metabolomics characterization, acini formation, proliferation, migration between different scaffolding compartments, and in vivo tumor formation. This model system provides an additional option for studying human breast diseases such as breast cancer.

Highlights

Hydrogel is a gel formed by networks of polymer chains, which have the ability to retain the water and gelatinize through various cross-linking processes
Homogenization of the fresh porcine breast fatty tissues directly obtained from local slaughter house was conducted in a precooled homogenizer, with the container holding the tissues embedded in ice and sliced tissues mixed with ice-cold water as described in the materials and methods
The homogenized tissues were decellularized with the non-ionic detergent triton X-100 or the zwitterionic detergent CHAPSO to remove the cellular contents while preserving the native and active states of the extracellular matrix (ECM) proteins

Summary

Introduction

Hydrogel is a gel formed by networks of polymer chains, which have the ability to retain the water and gelatinize through various cross-linking processes It has a wide range of applications ranging from biomedical research, tissue engineering, and clinical utilization to drug delivery [1,2,3,4]. The compositional differences of the substrata that cells live on are important for cell adhesion, migration, and growth since different cell surface receptors are expressed in response to their interacting proteins or particles within the matrices for extracellular and intracellular signaling and other biological functions To this end, collagen and the compositionally under-defined mouse sarcoma lrECM may induce cellular phenotypes different than those seen in cells grown on tissue-specific matrices since the levels and types of collagen and other components within ECM of different organs or tissues are quite distinct [12,13,14]

Methods

Results

Conclusion