Abstract
Acute liver failure (ALF) is a kind of complicated syndrome. Furthermore, adipose-derived mesenchymal stem cells (ADMSCs) can serve as a useful cell resource for autotransplantation due to their abundance and micro-invasive accessability. However, it is unknown how ALF will influence the characteristics of ADMSCs and whether ADMSCs from patients suffering from end-stage liver diseases are potential candidates for autotransplantation. This study was designed to compare various properties of ALF-derived ADMSCs with normal ADMSCs in pig models, with regard to their cellular morphology, cell proliferative ability, cell apoptosis, expression of surface antigens, mitochondrial and lysosomal activities, multilineage potency, and expression of liver-specific genes. Our results showed that ALF does not influence the stem cell characteristics and cell activities of ADMSCs. Intriguingly, the expression levels of several liver-specific genes in ALF-derived ADMSCs are higher than in normal ADMSCs. In conclusion, our findings indicate that the stem cell characteristics and cell activities of ADMSCs were not altered by ALF and these cells can serve as a new source for regenerative medicine.
Highlights
The liver is one of the few organs with an immense regeneration potential
We confirmed the Acute liver failure (ALF) group and normal group by liver histopathology, the morphological characteristics of ALF-derived adipose-derived mesenchymal stem cells (ADMSCs) and normal ADMSCs included a long spindle in clonogenic cells grown under culture conditions
Our results indicated that both ALF-derived porcine ADMSCs and normal porcine ADMSCs expressed mesenchymal markers but did not express hematopoietic markers
Summary
The liver is one of the few organs with an immense regeneration potential. At one time, the unique suitable therapy available for patients suffering from advanced or irreversible liver diseases was liver transplantation [1]. Adipose-derived mesenchymal stem cells (ADMSCs) demonstrated the longest culture period and the optimal proliferative ability compared with MSCs derived from other tissues [5]. Adipose tissue is likely to be an ideal source for less invasive procedures and may provide larger amounts of autologous stem cells than other types of MSCs. Regarding liver disease therapy, these cells can be used to treat hepatectomy [6], liver cirrhosis [7], liver failure [8], fulminant hepatitis [9] and other lethal liver diseases. The findings help us to clarify whether ADMSCs from patients with end-stage liver diseases can serve as a candidate source for cell autotransplantation
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