Abstract
Sclerotia of Phymatotrichum omnivorum were buried in nylon bags at a depth of 45 cm in Houston black clay at three sites in Temple, Texas. The crop history at each site was native prairie; agricultural rotation of cotton, corn and sorghum; or continuous cotton. Sclerotia were retrieved monthly and assessed for viability and associated fungi by washing, surface disinfesting, and then plating onto minimal salt agar, P. omnivorum sclerotium agar, or moistened filter paper. Total number of species and species diversity were significantly higher at the rotation site than at the prairie or cotton site. Percentage germination of sclerotia recovered from the cotton site was significantly greater than from the other two sites. Fusarium solani, Penicillium crustosum and P. purpurogenum were most frequently isolated from the rotation site, Gliocladium roseum and Trichoderma pseudokoningii from the prairie site and Aspergillus ustus from the continuous cotton site. Isolation medium did not significantly influence the total number of fungi isolated.
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