Populations of doubled haploids for genetic mapping in hexaploid winter triticale

M Tyrka,R J Metzger,A Ślusarkiewicz-Jarzina,A J Lukaszewski,J Rabiza-Swider,S Oleszczuk,M Wedzony,J Zimny,P S Baenziger,A Ponitka,H Wos

doi:10.1007/s11032-018-0804-3

Abstract

To create a framework for genetic dissection of hexaploid triticale, six populations of doubled haploid (DH) lines were developed from pairwise hybrids of high-yielding winter triticale cultivars. The six populations comprise between 97 and 231 genotyped DH lines each, totaling 957 DH lines. A consensus genetic map spans 4593.9 cM is composed of 1576 unique DArT markers. The maps reveal several structural rearrangements in triticale genomes. In preliminary tests of the populations and maps, markers specific to wheat segments of the engineered rye chromosome 1R (RM1B) were identified. Example QTL mapping of days to heading in cv. Krakowiak revealed loci on chromosomes 2BL and 2R responsible for extended vernalization requirement, and candidate genes were identified. The material is available to all parties interested in triticale genetics.

Highlights

Hexaploid triticale (×Triticosecale Wittm.), a wheat-rye hybrid with genomic constitution 2n = 6x = 42 (AABBRR) is slowly becoming an important cereal, already cultivated on about 4 million ha worldwide with over 70% of this area concentrated in the European Union (FAOSTAT 2014)
Triticale has been considered as bioenergy crop (Oettler et al 2003; Pronyk and Mazza 2011) and some consideration is given to the improvement of the bread-making properties (Lukaszewski 2006; Wos and Brzezinski 2015)
The starting materials were four cultivars and one breeding line of hexaploid winter triticale originating from various breeding programs

Summary

Introduction

Hexaploid triticale (×Triticosecale Wittm.), a wheat-rye hybrid with genomic constitution 2n = 6x = 42 (AABBRR) is slowly becoming an important cereal, already cultivated on about 4 million ha worldwide with over 70% of this area concentrated in the European Union (FAOSTAT 2014). Commercial importance of triticale is justified as it combines favorable agronomic characteristics of both parents, such as high yield potential and good grain quality of wheat with good adaptation to demanding growing conditions of rye. Breeding objectives of triticale focus on grain yield, adaptation towards biotic and abiotic stresses (Tyrka and Chełkowski 2004; Niedziela et al 2012), and development of hybrid cultivars (Góral 2002; Oettler et al 2003, 2005; Tams et al 2006; Stojałowski et al 2013). Triticale has been considered as bioenergy crop (Oettler et al 2003; Pronyk and Mazza 2011) and some consideration is given to the improvement of the bread-making properties (Lukaszewski 2006; Wos and Brzezinski 2015)

Methods

Results

Conclusion