Population structure of Euodia rutaecarpa in China revealed by amplified fragment length polymorphism (AFLP) and sequence-related amplified polymorphism (SRAP)

Abstract

In the present paper, experimental materials of 33 accessions representative of the Euodia rutaecarpa from four Chinese provinces were analyzed using the sequence-related amplified polymorphism (SRAP) and amplified fragment length polymorphism (AFLP) techniques, focusing on their molecular discrimination and the assessment of their genetic relatedness. For the analysis, we optimized 10 pairs of SRAP primers and 6 pairs of AFLP primers, (The software package NTSYS-pc 2.1 was applied to analyze the data matrix) and cluster analysis distributed samples into two clusters, one with E. rutaecarpa var. officinalis (Dode) Huang and the other with E. rutaecarpa (Juss.) Benth by SRAP+AFLP markers in the same similarity coefficient of 0.53 (Genetic parameters also analyzed using POPGENE version 1.31). Genetic diversity in the species was detected with SRAP (H = 0.2260, I = 0.3341) and AFLP (H = 0.1665; I = 0.2518) markers. Genetic variability levels of E. rutaecarpa var.officinalis (Dode) Huang was higher than genetic variability levels of E. rutaecarpa (Juss.) Benth. Our study shown that both SRAP and AFLP molecular markers are in high efficiency in detecting the genetic diversity of E. rutaecarpa. Key words: Euodia rutaecarpa, variety amplified fragment length polymorphism (AFLP) markers, sequence-related amplified polymorphism (SRAP) markers, genetic diversity.